Storage effects on whole blood: Physiological and electrical measurements

Abstract

Extracellular Na+, K+, pH, 2,3-DPG (diphosphoglycerate) and ATP and the comlex electrical impedance of whole blood samples from 31 male donors (21 donors form the training set and 10 donors were used for testing purpose) were measured on the 0th, 10th and 21st days of storage. Under storage, the extracellular fluid resistance (Re) and the intracellular fluid resistance (R i) decreased progressively with time; the effective cell membrane capacitance (Cm) has increased. Blood bank storage resulted in a rise in K+ and a fall in Na+, Cl-, pH, 2,3-DPG and ATP. Apart 2,3-DPG, electrical parameters were all correlated with the physiological parameters, at varying levels. By applying the multi-regression analysis, it is demonstrated that the parameters Ri, Re and Cm are appropriate for the assessment of physiological quality of blood until the 21st day of storage. © Springer-Verlag 2007.