RNA editing by A-to-I modification is a widespread mechanism in complex organisms that leads to the posttranscriptional alteration of protein coding as well as noncoding sequences. MiRNA transcripts have been recognized as a major target for RNA editing enzymes, and single-nucleotide changes through editing can impact the biogenesis of mature miRNAs, as well as the target specificity of the regulatory RNA. Bona fide A-to-I RNA editing events are validated experimentally through parallel analysis of genomic DNA and transcribed sequences of miRNA genes isolated from the same specimen through gene-specific amplification and sequencing of endogenous transcripts.
CITATION STYLE
Dupuis, D. E., & Maas, S. (2010). MiRNA editing. Methods in Molecular Biology (Clifton, N.J.), 667, 267–279. https://doi.org/10.1007/978-1-60761-811-9_18
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