Sesquiterpenoids from Aglaia cucullata Peel Fruit and Their Cytotoxic Activities Against B16-F10 and HeLa Cancer Cell Lines

Abstract

Sesquiterpenoids are terpenoid derivative compounds that have a diverse chemical structure and pharmacological effects. Sesquiterpenoids can be found in many plants of Aglaia which is the large source of natural compounds in the Meliaceae family. This investigation was intended to elucidate the structure of sesquiterpenoids from Aglaia cucullata peel fruit and their cytotoxicity against two human cancer cell lines. n-hexane extracts were separated by various chromatographic methods to yield three sesquiterpenoids. These sesquiterpenoids were identified by spectroscopy analysis (HR-TOFMS, IR,1H,13C-NMR, DEPT-135) as well as compared with spectral data which reported previously. The sesquiterpenoid compounds 1-3 were identified as spathulenol (1), alismol (2), and 10-oxoisodauc-3-en-15-al (3). The cytotoxic activity of three sesquiterpenoid compounds were tested against B16-F10 skin cancer cell and HeLa cervical cancer cell using the PrestoBlue method. Compound 2 exhibited the highest activity against both HeLa and B16-F10 cancer cell lines with IC50 218.33 μM and 258.90 μM, respectively.