Radioimmunoassay of prostaglandins in the semen of fertile men

Abstract

Specific antisera and iodinated tracers, as well as strict conditions of sample storage, PG extraction and purification, were used to estimate prostaglandins (μg/ml) in the semen of fertile men: 19-OH-PGE (1+2)(350), 19-OH-PGF-α (22), PGE-2 (15), PGE-1 (6.2), PGF-2α (2.3), PGF-1α (1.3), 6-keto-PGF-1α (<1), PGD-2, 13,14-dihydro-15-keto-PGFα and TXB-2 (<0.1). Only PGE-2 (50 pg/ml) was detectable in rat seminal fluid.