Enzyme activities in tissues and elimination half-lives of homologous muscle and liver enzymes in the racing pigeon (Columba livia domestica)

Abstract

Tissue enzyme profiles of heart, liver, pectoral muscle, quadriceps muscle, duodenum, kidney and brain from racing pigeons were established. The enzymes were alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), glutamate dehydrogenase (GLDH), lactate dehydrogenase (LDH), gamma glutamyltransferase (γ-GT), alkaline phosphatase (AP), and creatine kinase (CK). Elimination half-lives (t1/2β) of certain enzymes were also determined. The mean values (± SD) were: ASAT, 7.66 ± 1.55 (liver) and 6.51 ± 0.83 (muscle); ALAT, 15.69 ± 1.70 (liver) and 11.99 ± 1.32 (muscle); LDH, 0.71 ± 0.10 (liver) and 0.48 ± 0.07 (muscle); GLDH, 0.68 ± 0.17 (liver), CK 3.07 ± 0.59 hours (muscle). GLDH is the most liver-specific enzyme in the pigeon, but increased activities in the plasma are likely only in the acute stage of severe liver cell damage, since this enzyme is localised within the mitochondria and has a short half life. LDH and ASAT seem to be the most sensitive indicators of liver cell damage, though contributions come from muscle damage. Muscle cell damage can be differentiated from liver cell damage by measuring plasma CK activity, since CK is both a specific and a sensitive indicator of muscle cell damage. In a clinical setting the combined use of LDH, ASAT and CK permits differentiation between liver and muscle cell damage in racing pigeons. © 1988, Taylor & Francis Group, LLC. All rights reserved.