The critical operator determinants for λ repressor recognition have been defined by analyzing the binding of wild-type repressor to a set of mutant operators in vivo. Base pair substitutions at six positions within the λ operator half-site impair binding severely, and define these base pairs as critical for operator function. One mutant operator binds repressor better than the consensus operator, and is a superoperator. The model proposed by M. Lewis in 1983 for the binding of λ repressor to its operator accurately predicts the observed operator requirements for binding in vivo, with several minor exceptions. The order of affinities of the six natural λ operators has also been determined.
CITATION STYLE
Benson, N., Sugiono, P., & Youderian, P. (1988). DNA sequence determinants of λ repressor binding in vivo. Genetics, 118(1), 21–29. https://doi.org/10.1093/genetics/118.1.21
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