Transformation and Electrotransformation in Clostridia

Abstract

The introduction of DNA into bacteria by transformation remains an important step for the development of genetic and recombinant DNA technology. In addition to studying the structure and expression of exogenous genes either in Escherichia coli or Bacillus subtilis, it is important to reintroduce these genes in their original host. Genetic background, physiological, and environmental conditions are highly relevant to gene expression and regulation. This is particularly true for strict anaerobic bacteria such as Clostridia whose general physiology is very different than that of E. coli or B. subtilis. Further, doning foreign genes in strains of medical or economic significance with the purpose of modifying their genotype or improving their metabolic potentiality requires efficient gene transfer systems.