Trans-10, cis-12-conjugated linoleic acid modulates NF-κB activation and TNF-α production in porcine peripheral blood mononuclear cells via a PPAR-dependent pathway

Abstract

The activation of PPAR by ligands, including conjugated linoleic acid (CLA) isomers, plays an important role in the immune response. Among CLA isomers, trans-10, cis-12 (t10c12)-CLA is known to participate in the modulation of pro-inflammatory cytokine secretion. The aim of the present study was to assess the effect of t10c12-CLA on PPAR activation, NF-B activation and TNF- expression in lipopolysaccharide (LPS)-naive and LPS-stimulated porcine peripheral blood mononuclear cells (PBMC). In addition, the effect of PPAR inhibition on NF-B activation and TNF- expression in porcine PBMC was examined. t10c12-CLA was found to increase TNF- expression and NF-B activity in LPS-naive porcine PBMC. In contrast, t10c12-CLA decreased TNF- expression and NF-B activity in LPS-stimulated porcine PBMC. t10c12-CLA up-regulated PPAR activity and mRNA expression in both LPS-naive and LPS-stimulated porcine PBMC. GW9662, a PPAR antagonist, completely negated the modulating effects of t10c12-CLA on TNF- expression and NF-B activity in both LPS-naive and LPS-stimulated porcine PBMC. These results suggest that t10c12-CLA can modulate TNF- production and NF-B activation by a PPAR-dependent pathway in porcine PBMC. © 2011 The Authors.