The ATP-binding cassette (ABC) transporters form one of the largest known-protein families; these are widespread in bacteria, archaea, and eukaryotes and are encoded in the largest set of paralogous genes. In the Synechocystis sp. PCC6803 genome, over 50 ABC transporter-related genes have been detected by genome sequence analysis. Some of these have been identified as Na+/H+, iron, phosphate, polysaccharide, and CO2 transporters. The substrates of many other ABC transporters are still unknown. To identify ABC transporters involved in acid tolerance, deletion mutants of other substrate-unknown ABC transporter genes were screened for their acid-stress sensitivities in a low-pH medium. A mutant of slr1045 was found to be more sensitive to acid stress than wild-type cells. The abundance of expression of the genes was analyzed under various stress conditions by quantitative real time reverse transcriptase-polymerase chain reaction.
CITATION STYLE
Tahara, H., Fukai, S., Sambe, M., Kobayashi, M., Uchiyama, J., & Ohta, H. (2013). Characterization of the ABC transporter gene slr1045 involved in acid-stress tolerance of synechocystis sp. PCC 6803. In Advanced Topics in Science and Technology in China (pp. 596–598). Springer Science and Business Media Deutschland GmbH. https://doi.org/10.1007/978-3-642-32034-7_129
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