Multiple inducible cytochromes P-450 in yeast.

Abstract

A form of cytochrome P-450 is produced in Saccharomyces cerevisiae during the logarithmic growth phase. This form is inhibited by metyrapone, tetrahydrofuran, and carbon oxide which are inhibitors of ethanol-inducible cytochromes P-450 of mammals. Addition of Na-phenobarbital to the 0.5% glucose liquid medium induced a form of cytochrome P-450 inhibited only by metyrapone. Thus, yeast possess multiple forms of cytochrome P-450 that are individually inducible.