Handbook of Histopathological Technique

Abstract

Third edition. Handbook of Histopathological and Histochemical Techniques (Including Museum Techniques), Third Edition is a reference guide on techniques used in histology and histochemistry, including museum techniques. This book is comprised of 36 chapters divided into six sections. After giving a brief description of the structure and contents of the cell, this book then turns to the various methods of examination of tissue cells and structures, including the smear method, vital staining, and electron microscopy. The next two sections deal with fixation, decalcification, processing, and section cutting, a. Front Cover; Handbook of Histopathological and Histochemical Techniques: Including Museum Techniques; Copyright Page; Table of Contents; Foreword; Preface to the Third Edition; Preface to the First Edition; Acknowledgements; Part I: Introductory; Chapter 1. The Cell; THE NUCLEUS; THE CYTOPLASM; CELL DIVISION; COLLOIDAL CONCEPTION OF TISSUE; OSMOSIS; Chapter 2. Methods of Examination of Tissues and Cells; DISSOCIATION; SMEAR TECHNIQUE; THICK SECTION; VITAL STAINING; SECTIONAL METHODS; FLUORESCENT ANTIGEN-ANTIBODY TECHNIQUES; POLARIZING MICROSCOPE; DARK GROUND ILLUMINATION. PHASE-CONTRAST MICROSCOPYINTERFERENCE MICROSCOPY; ULTRA-VIOLET LIGHT MICROSCOPY; FLUORESCENT MICROSCOPY; ELECTRON MICROSCOPY; MICRO-INCINERATION; MICRO-INCINERATION; Part II: Fixation, Processing and Section Cutting; Chapter 3. Fixation; INTRODUCTION; THE AIMS AND EFFECTS OF FIXATION; REAGENTS EMPLOYED AS FIXATIVES; COMPOUND FIXATIVES; MICRO-ANATOMICAL FIXATIVES; CYTOLOGICAL FIXATIVES; HISTOCHEMICAL FIXATIVES; VAPOUR FIXATIVES; POST-CHROMATIZATION; FREEZE DRYING; FREEZE SUBSTITUTION; Chapter 4. Decalcification; DECALCIFYING FLUIDS; USE OF ION-EXCHANGE RESINS; CHELATING AGENTS. ELECTROPHORETIC DECALCIFICATIONTREATMENT OF HARD TISSUES; Chapter 5. Processing; PARAFFIN WAX EMBEDDING; RAPID PARAFFIN PROCESSING OF TISSUES; ESTER WAX EMBEDDING; WATER-SOLUBLE WAXES; GELATIN EMBEDDING; CELLOIDIN EMBEDDING; LOW VISCOSITY NITRO-CELLULOSE (L.V.N.); THE DRY CELLOIDIN METHOD; DOUBLE EMBEDDING; Chapter 6. Section Cutting; MICROTOME KNIVES; MICROTOMES; CUTTING OF PARAFFIN WAX EMBEDDED SECTIONS; CUTTING PARAFFIN WAX EMBEDDED SECTIONS OF DIFFICULT TISSUE; CUTTING SERIAL SECTIONS; CUTTING OF CELLOIDIN EMBEDDED SECTION; FROZEN SECTIONS; CRYOSTAT CUT SECTIONS. Part III: Stains, Impregnations and MountantsChapter 7. Theory of Staining; STAINS AND DYES; METHODS OF USE; PRINCIPLES OF DYE CHEMISTRY; CLASSIFICATION OF DYES; MORDANTS; ACCENTUATORS; METACHROMASY; THE USE OF CONTROL SECTIONS IN THE IDENTIFICATION OF CHEMICAL STRUCTURES OR TISSUE ELEMENTS; Chapter 8. Preparation of Stains; SOLVENTS; BUFFER TABLES; Chapter 9. Mountants; AQUEOUS MOUNTING MEDIA; RESINOUS MOUNTING MEDIA; RINGING MEDIA; Chapter 10. Basic Staining and Mounting Procedures; EQUIPMENT; THE APPLICATION OF HEAT DURING STAINING PROCEDURES; PARAFFIN SECTIONS; FROZEN SECTIONS. CELLOIDIN SECTIONSSTAINING SERIAL SECTIONS OF CELLOIDIN-IMPREGNATED MATERIAL; RAPID FROZEN SECTIONS FOR EMERGENCY DIAGNOSES; Chapter 11. Routine Stains; HAEMATOXYLIN AND EOSIN; HAEMATOXYLINS; COUNTERSTAINS ROUTINELY EMPLOYED WITH HAEMATOXYLIN NUCLEAR STAINS; EOSIN-AZURE STAINS (ROMANOWSKY STAINS); SINGLE STAINS; Chapter 12. Proteins; INTRODUCTION; CHEMISTRY; IDENTIFICATION OF PROTEINS; GENERAL METHODS FOR PROTEIN; METHODS FOR SPECIFIC GROUPS OR AMINO ACIDS; METHODS FOR SULPHYDRYL GROUPS (SH); METHODS FOR DISULPHIDE GROUPS ( -SS- ); METHODS FOR CARBOXYL GROUPS (COOH).