Detecting Blue Light-Dependent Protein–Protein Interactions by LexA-Based Yeast Two-Hybrid Assay

Abstract

The LexA-based yeast two-hybrid system is one of the most powerful techniques used to detect blue light-dependent protein–protein interactions. In Arabidopsis, many protein–protein interactions in blue light signaling pathway were identified using this system. Here we present an easy and efficient method of the LexA-based yeast two-hybrid assay for testing protein–protein interactions in a blue light-dependent manner.