The potential of Rhizophora mucronata extracts to protect tiger prawn from pathogenenic infections

Abstract

The research aims to study Rhizophora mucronata leaves extract to inhibit and protects tiger prawn from pathogens infection. The leaves of R. mucronata were chopped, dried, macerated and extracted in three different solvents, namely ethanol, water and see water. In vitro inhibitory tests were performed using disc diffusion and minimal inhibitory concentration methods. The in vivo test on tiger prawn was given by submersion, which then tested with Vibrio harveyi and Saprolegnia sp. The observation and inspection covered clinical symptom, pathological anatomy, total vibrio count, survival rate, and Relative Percentage of Survival. The result showed that mangrove leaves extract can inhibit pathogens on tiger prawn, improve survival rate and relative persentage of survival. The best R. mucronata extract that can inhibit and protect the tiger prawn from pathogen infection is ethanol extract 1,250-1,500 ppm, followed by water extract 1,500 ppm, and sea water extract 1,500 ppm.