Purpose: To investigate the effect of microenvironment maintenance on embryo culture and clinical results using two types of incubators. Methods: Temperature and oxygen concentration in a mini-incubator and a conventional incubator were compared following a 5-s door opening/closing procedure. Embryos of 30 in-vitro fertilization embryo transfer (IVF-ET) cases were randomly allocated to either one of the incubator, cultured, and the early-stage good embryo formation rate and the good blastocyst formation rate were compared, as indicators for micro-environment maintenance ability. Results: Temperature recovery after a 5-s door opening/closing procedure was approximately 5 min for the mini-incubator and 30 min for the conventional incubator. The oxygen concentration return was significantly improved in the mini-incubator (3.0 ± 0 min) compared with the conventional incubator (7.8 ± 0.9 min). Both the early-stage good embryo formation rate and the good blastocyst formation rate were significantly higher in the mini-incubator (39.5% and 15.1%) than the conventional incubator (28.4% and 7.8%). Conclusion: The microenvironment maintenance ability of incubators appears to significantly influence the formation of good embryos. © 2006 Springer Science+Business Media, LLC.
CITATION STYLE
Fujiwara, M., Takahashi, K., Izuno, M., Duan, Y. R., Kazono, M., Kimura, F., & Noda, Y. (2007). Effect of micro-environment maintenance on embryo culture after in-vitro fertilization: Comparison of top-load mini incubator and conventional front-load incubator. Journal of Assisted Reproduction and Genetics, 24(1), 5–9. https://doi.org/10.1007/s10815-006-9088-3
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