Chromatographic fingerprint analysis of piperine in polyherbal and marketed formulation by hptlc and GC-MS methods

Abstract

The standardization of polyherbomineral formulation (PHF) is significant with regard to accessthe quality of natural medicines. The current research study highlights the chromatographic fingerprint investigation of piperine in PHF by employing GC-MS and HPTLC. PHF contain piperinewhich is utilized to take care of cough and cold. It was prepared from the mixture of Zingiber officinalis (Ginger), Piper nigrum (Kali mirch), Piper longum (Pipali), Terminalia belerica (Bahera), Terminalia chebula (Harde), Cuminumcyminum (Jira), Piper retrofractum (Chavya), Emblica officinalis (Amla), Coriander sativum (Dhaniya), sulphur, mercury, abharakbhasam and lohbhasam. The methanolic extract of both PHF and market formulation (MF) were subjected to HPTLC and GC-MS chromatographic analysis. HPTLC chromatogram finger printing of piperine in PHF demonstrated Rf values at 0.49 which was found in MF (Rf 0.47) and in standard marker (Rf 0.43). The piperine phytoconstituent present in both MF and PHF were investigated and recognized by GC-MS analysis, Thin layer chromatography (TLC), Fourier transformer infra-red (FTIR) spectroscopy and phytochemical tests. HPTLC finger printing, GC-MS analysis, FTIR and phytochemical screening tests of PHF may be useful in discriminating the species, affirm the existence of piperine phytoconstituen and act as a biochemical marker for polyherbomineral formulation. The consequence of these acquired parameters could serve as diagnostic tools to assist the regulatory authorities, scientific manufacturers and organizations for authentication and growing standard polyherbomineral formulation of high efficacy.