Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system (CNS) often used as a model for the early inflammatory stages of multiple sclerosis and also as a model of organ-specific autoimmune disease. This protocol describes the induction of passive EAE in mice, either using T cells isolated from mice primed with myelin antigens, or through the use of naïve TCR transgenic T cells activated in vitro in the presence of myelin-derived antigens.
CITATION STYLE
McPherson, R. C., Cambrook, H. E., O’Connor, R. A., & Anderton, S. M. (2014). Induction of passive EAE using myelin-reactive CD4+ T cells. Methods in Molecular Biology, 1193, 187–198. https://doi.org/10.1007/978-1-4939-1212-4_17
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