The luciferase immunoprecipitation system (LIPS) assay is a liquid-phase immunoassay that quantitates antigen-specific serum antibodies by measuring luminescence emitted by the reporter enzyme Renilla luciferase (Ruc) fused to an antigen of interest. The LIPS assay can be utilized as a high-throughput and sensitive serological method for profiling serum antibodies recognizing diverse antigens. This chapter provides a detailed protocol for detecting human norovirus-specific serum antibodies with the LIPS assay.
CITATION STYLE
Tin, C. M., & Sosnovtsev, S. V. (2019). Detection of Human Norovirus-Specific Antibodies Using the Luciferase Immunoprecipitation System (LIPS) Assay. In Methods in Molecular Biology (Vol. 2024, pp. 137–152). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9597-4_8
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