Protein microarray is considered to be one of the key analytical tools for high-throughput protein function analysis. We found that Arabidopsis HY5 protein functions as a novel DNA-binding tag (DBtag), and DBtagged proteins are immobilized and purified on a newly designed agarose/DNA microplate. In this chapter, we demonstrate a protocol for making the DBtag-based protein microarray and will provide protocols for two applications using the microarray: (1) detection of autophosphorylation activity of DBtagged human protein kinases and inhibition of their activity by staurosporine, and (2) detection of a protein-protein interaction between the DBtagged UBE2N and UBE2v1.
CITATION STYLE
Sawasaki, T., & Endo, Y. (2010). Cell-free-based protein microarray technology using agarose/DNA microplate. Methods in Molecular Biology (Clifton, N.J.), 607, 63–72. https://doi.org/10.1007/978-1-60327-331-2_7
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