Detection of mannophosphoinositide antigens in sputum of tuberculosis patients by dot enzyme immunoassay

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Abstract

A simple and economical dot ELISA for the detection of mannophosphoinositide antigen in sputum samples of tuberculosis patients has been developed using affinity-purified antibodies. This test is able to detect free as well as bound antigen. Sputum samples from 94 patients suffering from tuberculosis and 30 non-tuberculosis patients were screened and an overall sensitivity and specificity of 89% and 93.3%, respectively, was obtained. The sensitivity of the test among the different groups of tuberculosis patients did not vary significantly. © 1991 Springer-Verlag.

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Kansal, R., & Khuller, G. K. (1991). Detection of mannophosphoinositide antigens in sputum of tuberculosis patients by dot enzyme immunoassay. Medical Microbiology and Immunology, 180(2), 73–78. https://doi.org/10.1007/BF00193848

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