Lacasse catalyzed-synthesis of 4,4′-biphenyldiamine from P-chloroaniline. Evaluation of antifungal and antioxidant activities

Abstract

Laccase is a copper-containing oxidase that catalyzes reduction of molecular oxygen to water and the oxidation of a phenolic compound. In this paper, laccase was utilized to synthesize 4,4′-Biphenyldiamine using p-chloroaniline as substrate by means of a coupling reaction. The synthesized compound, 4,4′-Biphenyldiamine, presented low antifungal activity against the phytopathogenic fungus Botrytis cinerea, however the antioxidant ability, measurement by ORAC-PGR method, was higher than substrate. This work corresponds to the first report of synthesis 4,4′-Biphenyldiamine, from p-chloroaniline in a lacasse-catalyzed reaction.