Abstract
Cellulases and other enzymes are needed for saccharification of plant biomass in the biorefinery industry. Expression, characterization, and eventual large-scale production of known and novel cellulases requires the ability to express and secrete heterologous enzymes in relevant protein production platforms like Aspergillus niger. A method for cloning and expression of genes for these desirable enzymes in A. niger is presented in this Chapter.
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Reilly, M. C., Campen, S. A., Simmons, B. A., Baker, S. E., Gladden, J. M., & Magnuson, J. K. (2018). Cloning and expression of heterologous cellulases and enzymes in aspergillus niger. In Methods in Molecular Biology (Vol. 1796, pp. 123–133). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7877-9_10
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