Efeito de 6-benzilaminopurina sobre a propagação in vitro de mimosa caesalpiniifolia benth. (Fabaceae)

Abstract

The Mimosa caesalpiniifolia, popularly known as sabiá, is a native species belonging to Caatinga forest that due to its countless potentialities has faced a process of exhaustive exploration that makes imminent the need to use sustainable alternatives to its replacement in natural environment and the conservation of its genotype. Accordingly, micropropagation has been considered as a promising technique, since it allows the production of seedlings in large scale and high. The cytokinins, such as 6-benzylaminopurine (BAP), are important in this process, since they influence considerably the growth and morphogenesis in vitro morphogenesis allowing the formation on in vitro germplasm banks. The aim of this study was to investigate the influence of different concentrations of BAP on in vitro budding induction of M. caesalpiniifolia. Cotyledon segments obtained from in vitro germinated seedlings were inoculated in WPM supplemented with six BAP concentrations: 0.0; 4.44; 8.88; 17.76; 26.64 and 35.52 μmol/L. The experimental design was randomized with 6 treatments, 3 replications and each replication consisting of 10 experimental units. After 30 days, the following parameters have been evaluated: number of responsive explants, number of sprouts per explant and presence of callus, oxidation and contamination. The data were statistically analyzed by Correspondence Analysis and through adjusting Regression Equations. It was found that the concentration of 17.76 μmol/L was more responsive with regard to multiplication rate and sprouts number. In this case, is the most suitable concentration for the in vitro propagation of M. caesalpiniifolia.