Anti Genotoxic Effect of TiO2 Nanoparticle Biosynthesized from Sargassum polycystum - a Marine Macroalgae

Abstract

Nanotechnology is the application of science and technology to control matter at the molecular level, which is also referred to as the ability for designing, production, characterization and application to structures, devices and systems by controlling shape and size at the nanometer scale The present study was an attempt to investigate antigenotoxic effect of TiO2 nanoparticle biosynthesized from Sargassum polycystum Our work aimed at conducting Genotoxicity studies for the screening of Mutagenicity using Ames test and to prove this as a antigenotoxicant. The mutagens (positive controls) treated without metabolic activation system showed a 3 fold increase of average revertant colonies per plate when compared with that of concurrent vehicle controls, thus exhibiting the ability to identify the mutagen by the tester strains. The mutagenicity assay was performed with three dose levels (0.312, 1.25, and 5.0 mg/ml) in the absence of metabolic activation system. Inhibition of background growth of non-revertant bacteria was not found at any of the three dose levels. Furthermore works are to be carried out in future to find the exact mechanism of its genoprotective nature;