In Vivo Investigation of High-Fat Diet–Induced Hepatic Lipid Dysfunctions

Abstract

Fat distribution, on top of general obesity, contributes to the severity of histologic features in patients with nonalcoholic fatty liver diseases (NAFLD); and visceral obesity has been correlated to fatty liver diseases. Therefore, investigation of fat distribution in vivo could be a good predictor of fatty liver risks in obesity. Fatty acids composition is a key player in hepatic dysfunctions and cardiovascular risk in obesity. Because fatty acids can damage biological membranes, fatty acid accumulation in the liver may be partially responsible for the functional and morphological changes that are observed in NAFLD. Fatty acids stored into triglycerides are lipid species that act as signaling molecules and therefore are key regulators of posttranslational regulation of biological functions such as lipid homeostasis and lipotoxicity. Here, we describe magnetic resonance methods to investigate in vivo whole-body fat distribution and hepatic liver fatty acid composition in order to directly assess the liver metabolic status and may allow to anticipate liver diseases.