A novel chitosanase from Bacillus sp. strain K17: gene cloning and expression in Escherichia coli.

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Abstract

The gene encoding a novel chitosanase from Bacillus sp. strain K17 was cloned and sequenced. The nucleotide sequence of the gene contained an open reading frame corresponded to a protein of 453 amino acids. The deduced amino acid sequence of the K17 chitosanase exhibited the highest homology to those of family 8 glycanases, suggesting that the enzyme belonged to family 8.

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Yatsunami, R., Sakihama, Y., Suzuki, M., Fukazawa, T., Shimizu, S., Sunami, T., … Nakamura, S. (2002). A novel chitosanase from Bacillus sp. strain K17: gene cloning and expression in Escherichia coli. Nucleic Acids Research. Supplement (2001), (2), 227–228. https://doi.org/10.1093/nass/2.1.227

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