To mutagenize rice genomes, a two-element system is utilized. This system comprises an immobile Ac element driven by the CaMV 35S promoter, and a gene trap Ds carrying a partial intron with alternative splice acceptors fused to the GUS coding region. Rapid, large-scale generation of a Ds transposant population was achieved using a plant regeneration procedure involving the tissue culture of seed-derived calli carrying Ac and Ds elements. During tissue cultures, Ds mobility accompanies changes in methylation patterns of a terminal region of Ds, where over 70 % of plants contained independent Ds insertions. In the transposon population, around 12 % of plants expressed GUS at the early seedling stage. A flanking-sequence-tag (FST) database has been established by cloning over 19,968 Ds insertion sites and the Ds map shows relatively uniform distribution across the rice chromosomes. © Springer Science+Business Media, New York 2013.
CITATION STYLE
Xuan, Y. H., Huang, J., Yi, G., Park, D. S., Park, S. K., Eun, M. Y., … Han, C. D. (2013). Plant regeneration methods for rapid generation of a large scale Ds transposant population in rice. Methods in Molecular Biology, 1057, 101–116. https://doi.org/10.1007/978-1-62703-568-2_7
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