Differential expression of dehydrin in flower buds of two Japanese apricot cultivars requiring different chilling requirements for bud break

Abstract

In this study, we investigated seasonal changes in protein profiles in dormant flower buds of Japanese apricot (Prunus mume Siebold & Zucc.) cultivars 'Ellching', from subtropical Taiwan, and 'Nanko', from temperate Japan. One protein, isolated by two-dimensional polyacrylamide gel electrophoresis of flower bud extracts, was shown by peptide sequencing to be a dehydrin (the group of D-11 LEA (late embryogenesis-abundant) proteins). Patterns of dehydrin protein and transcript accumulation differed between the cultivars, with greater accumulations and longer persistence in 'Nanko' than in 'Ellching'. These differences correspond with the greater requirement for chilling to break flower bud dormancy in 'Nanko' than in 'Ellching'. Our study supports the findings of earlier work comparing dehydrin expression in the bark tissue of the evergreen and deciduous peach (Prunus persica (L.) Batsch) genotypes, and suggests that the role of dehydrin during the dormant season is common to all Prunus species. © 2006 Heron Publishing.