We automated the Jendrassik-Grof method for measurement of bilirubin with the Greiner Selective Analyzer GSA II D. Comparison with the manual Jendrassik-Grof method showed a highly significant correlation coefficient of 0.994 for total and 0.980 for 'direct' bilirubin measurement. The automated method showed good precision and was linear to 667 μmol/L, the highest concentration tested. Addition of hemolysate caused a greater effect on the measurement of direct bilirubin than on total bilirubin. The inhibitory effect on measurement of total bilirubin, though small, was statistically significant at hemoglobin concentrations >2 g/L. Comparison with the method involving diazotized 2,4-dichloroaniline showed the Jendrassik-Grof method to be relatively less affected by hemolysis and turbidity in the measurement of total bilirubin.
Mendeley helps you to discover research relevant for your work.
CITATION STYLE
Cherian, A. G., Soldin, S. J., & Hill, J. G. (1981). Automated Jendrassik-Grof method for measurement of bilirubin in serum with the Greiner Selective Analyzer (GSA IID), and comparison with the method involving diazotized 2,4-dichloroaniline. Clinical Chemistry, 27(5), 748–752. https://doi.org/10.1093/clinchem/27.5.748