A rapid “on-off-on” mitochondria-targeted phosphorescent probe for selective and consecutive detection of Cu2+and cysteine in live cells and zebrafish

Abstract

The detection of mitochondrial Cu2+and cysteine is very important for investigating cellular functions or dysfunctions. In this study, we designed a novel cyclometalated iridium(iii) luminescence chemosensor Ir bearing a bidentate chelating pyrazolyl-pyridine ligand as a copper-specific receptor. The biocompatible and photostable Ir complex exhibited not only mitochondria-targeting properties but also an “on-off-on” type phosphorescence change for the reversible dual detection of Cu2+and cysteine. Ir had a highly sensitive (detection limit = 20 nM) and selective sensor performance for Cu2+in aqueous solution due to the formation of a non-phosphorescent Ir-Cu(ii) ensemble through 1 : 1 binding. According to the displacement approach, Ir was released from the Ir-Cu(ii) ensemble accompanied with “turn-on” phosphorescence in the presence of 0-10 μM cysteine, with a low detection limit of 54 nM. This “on-off-on” process could be accomplished within 30 s and repeated at least five times without significant loss of signal strength. Moreover, benefiting from its good permeability, low cytotoxicity, high efficiency, and anti-interference properties, Ir was found to be suitable for imaging and detecting mitochondrial Cu2+and cysteine in living cells and zebrafish.