Local circuit synaptic interactions in hippocampal brain slices

Abstract

The hypothesis that recurrent inhibition in the hippocampus is mediated by inhibitory interneurons was tested with simultaneous intracellular recordings from the CA1 region of guinea pig hippocampus slices in vitro. In recordings from 101 pairs of pyramidal cells, no interactions were detected in 87% of the pairs. In 13% of the pyramidal cell pairs, spike trains induced in one cell caused inhibitory postsynaptic potentials (IPSPs) in the second cell. No excitatory interactions were detected. In recordings from 43 pairs of cells, where one cell was a pyramidal cell and the other cell was an interneuron, no interactions were detected in 53% of the pairs. In 30% of the interneuron-pyramidal cell pairs, spike trains elicited from the interneuron caused IPSPs in the pyramidal cell. In 29% of the pairs, spike trains elicited from the pyramidal cell caused excitatory postsynaptic potentials (EPSPs) in the interneurons. In 4% of these pairs, reciprocal interactions were seen with the pyramidal cell exciting the interneuron and the interneuron inhibiting the pyramidal cell. These results support the hypothesis that inhibitory interneurons mediate recurrent inhibition in the hippocampus. However, the data also suggest that the interneurons from which these results were recorded were a subset of inhibitory interneurons distinct from the classical basket cell. These interneurons may mediate both feed-forward and recurrent inhibition in the hippocampus.