Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale analysis and our protocol worked well on formalin-fixed, paraffin-embedded samples. © 2010 Nature America, Inc. All rights reserved.
CITATION STYLE
Gu, H., Bock, C., Mikkelsen, T. S., Jäger, N., Smith, Z. D., Tomazou, E., … Meissner, A. (2010). Genome-scale DNA methylation mapping of clinical samples at single-nucleotide resolution. Nature Methods, 7(2), 133–136. https://doi.org/10.1038/nmeth.1414
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