The utility of reporter genes has gained significant momentum over the last three decades. Reporter genes are used to understand the transcriptional activity of a gene both in vitro and in vivo, and in pathway analysis and drug screening for diseases involving protozoan parasites, and in anti-cancer drug developments. Here, using a human prostate cancer xenograft model (PC3), we describe a method to construct and validate hypoxia reporter genes with different half-lives. Using molecular biology and optical imaging techniques, we have validated the expression of long half-life enhanced green fluorescence protein (EGFP) expression and short half-life luciferase gene expression to report on the spatial and temporal evolution of hypoxia in vivo.
CITATION STYLE
Krishnamachary, B., Danhier, P., Kakkad, S., Bharti, S. K., & Bhujwalla, Z. M. (2018). Hypoxia-induced reporter genes with different half-lives. In Methods in Molecular Biology (Vol. 1790, pp. 113–125). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7860-1_9
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