Identification of a urine metabolite constellation characteristic for kidney allograft rejection

Abstract

Introduction: Allograft rejection is still an important complication after kidney transplantation. Currently, monitoring of these patients mostly relies on the measurement of serum creatinine and clinical evaluation. The gold standard for diagnosing allograft rejection, i.e. performing a renal biopsy is invasive and expensive. So far no adequate biomarkers are available for routine use. Objectives: We aimed to develop a urine metabolite constellation that is characteristic for acute renal allograft rejection. Methods: NMR-Spectroscopy was applied to a training cohort of transplant recipients with and without acute rejection. Results: We obtained a metabolite constellation of four metabolites that shows promising performance to detect renal allograft rejection in the cohorts used (AUC of 0.72 and 0.74, respectively). Conclusion: A metabolite constellation was defined with the potential for further development of an in-vitro diagnostic test that can support physicians in their clinical assessment of a kidney transplant patient.