E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells

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Abstract

Here we present data supporting the role of lipid rafts in endothelial cells during leukocyte adhesion. Following adhesion of THP-1 cells or antibody-mediated clustering, both E-selectin and intercellular adhesion molecule-1 (ICAM-1) partitioned into the detergent-insoluble portion of the endothelial cellular lysate. Sucrose gradient centrifugation revealed the partitioning of clustered E-selectin and ICAM-1 with the low-density fraction where they co-fractionated with src family kinases, markers of lipid rafts. Depleting the plasma membrane of cholesterol inhibited clustering of adhesion molecules following their antibody-induced crosslinking and inhibited their association with src kinases. Thus, our data suggest that E-selectin and ICAM-1 associate with lipid rafts in human endothelial cells following leukocyte adhesion. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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Tilghman, R. W., & Hoover, R. L. (2002). E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells. FEBS Letters, 525(1–3), 83–87. https://doi.org/10.1016/S0014-5793(02)03070-3

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