Expression Stabilities of Candidate Reference Genes for RT-qPCR under Different Stress Conditions in Soybean

56Citations
Citations of this article
40Readers
Mendeley users who have this article in their library.

Abstract

Due to its accuracy, sensitivity and high throughput, real time quantitative PCR (RT-qPCR) has been widely used in analysing gene expression. The quality of data from such analyses is affected by the quality of reference genes used. Expression stabilities for nine candidate reference genes widely used in soybean were evaluated under different stresses in this study. Our results showed that EF1A and ACT11 were the best under salinity stress, TUB4, TUA5 and EF1A were the best under drought stress, ACT11 and UKN2 were the best under dark treatment, and EF1B and UKN2 were the best under virus infection. EF1B and UKN2 were the top two genes which can be reliably used in all of the stress conditions assessed. © 2013 Ma et al.

Cite

CITATION STYLE

APA

Ma, S., Niu, H., Liu, C., Zhang, J., Hou, C., & Wang, D. (2013). Expression Stabilities of Candidate Reference Genes for RT-qPCR under Different Stress Conditions in Soybean. PLoS ONE, 8(10). https://doi.org/10.1371/journal.pone.0075271

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free