Identifying the m6A methylome by affinity purification and sequencing

Abstract

N6-methyladenosine (m6A) is the most abundant internal modification in eukaryotic mRNA, and is newly emerging as a key posttranscriptional mRNA regulator. Recent research has uncovered insight into the location and function of m6A sites on a large scale, in part due to the transcriptome-wide identification of m6A sites by high-throughput sequencing (m6A-seq). Here, we present a protocol for m6A-seq, which maps the m6A methylome by affinity purification and sequencing.