Characterisation of cauliflower mosaic virus DNA sequences which encode major polyadenylated transcripts

Abstract

Cauliflower mosaic virus DNA sequences which encode two major polyadenylated RNA species, the 1.9 kb messenger RNA for the 62,000 MW virus inclusion body polypeptide and 355 RNA, were mapped using the nuclease S1 procedure. The 1.9 kb RNA has an eleven nucleotide leader sequence transcribed from a-strand DNA located at co-ordinate 0.72 m.u. (map units), immediately upstream of the AUG initiation codon of reading frame VI. The 3′-end of 1.9 kb RNA maps at 0.95 m.u. and is co-terminal with the 3′-end of 355 RNA, a complete transcript of the DNA α-strand. The 5′-end of 355 RNA maps at 0.93 m.u. and is located upstream of the discontinuity G1 (zero m.u.) in the α-strand and some 200 nucleotides upstream of the sequence encoding its own 3′-terminus. © 1981 IRL Press Limited.