Study of Cerebellum Structure and Histomorphometry of Local Chicken ( Gallus gallus domesticus ) Before and After Hatching

Abstract

Cerebellum is an organ that plays an important role in controlling and coordinating motoric nerves and maintaining body balance. This study aimed to observe the histological and histomorphometric structures of the cerebellum in native chickens before and after hatching. The samples of this study were divided into four groups of different age, namely K1(7th day of incubatiuon), K2 (14th day of incubation), K3 (20th day of incubation), and K4 (7th day after hatching). Each group consists of six chicken cerebellum. Based on the results of macroscopic observations, the size of cerebellum was increased in each group. Histologyobservation showed that in K1, substantiagrisea was present consisting of molecular and granular layers. In K2, substantia alba has been formed, and in K3 and K4, a Purkinje layer was established. Histomorphometry examination showed that substantia alba in each group was decreased, however the size of substantia alba in K3 was not significantly different (P> 0.05) from K4. Substantia grisea has undergone a significant increase (P <0.05) in each group. It can be concluded that the histological structure of the cerebellum in each age group has developed. In histomorphometric measurements, the substantia alba was decreased in each group. However, the substantiagrisea and its layers has increased in all grups.