Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella

159Citations
Citations of this article
74Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

The inner dynein arm regulates axonemal bending motion in eukaryotes. We used cryo-electron tomography to reconstruct the three-dimensional structure of inner dynein arms from Chlamydomonas reinhardtii. All the eight different heavy chains were identified in one 96-nm periodic repeat, as expected from previous biochemical studies. Based on mutants, we identified the positions of the AAA rings and the N-terminal tails of all the eight heavy chains. The dynein f dimer is located close to the surface of the A-microtubule, whereas the other six heavy chain rings are roughly colinear at a larger distance to form three dyads. Each dyad consists of two heavy chains and has a corresponding radial spoke or a similar feature. In each of the six heavy chains (dynein a, b, c, d, e, and g), the N-terminal tail extends from the distal side of the ring. To interact with the B-microtubule through stalks, the inner-arm dyneins must have either different handedness or, more probably, the opposite orientation of the AAA rings compared with the outer-arm dyneins. © 2008 Bui et al.

Cite

CITATION STYLE

APA

Bui, K. H., Sakakibara, H., Movassagh, T., Oiwa, K., & Ishikawa, T. (2008). Molecular architecture of inner dynein arms in situ in Chlamydomonas reinhardtii flagella. Journal of Cell Biology, 183(5), 923–932. https://doi.org/10.1083/jcb.200808050

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free