Whole-genome sequencing of aggregatibacter species isolated from human clinical specimens and description of Aggregatibacter kilianii sp. Nov.

Abstract

Aggregatibacter species are commensal bacteria of human mucosal surfaces that are sometimes involved in serious invasive infections. During the investigation of strains cultured from various clinical specimens, we encountered a coherent group of 10 isolates that could not be allocated to any validly named species by phenotype, mass spectrometry, or partial 16S rRNA gene sequencing. Whole-genome sequencing revealed a phylogenetic cluster related to but separate from Aggregatibacter aphrophilus. The mean in silico DNA hybridization value for strains of the new cluster versus A. Aphrophilus was 56% (range, 53.7 to 58.0%), whereas the average nucleotide identity was 94.4% (range, 93.9 to 94.8%). The new cluster exhibited aggregative properties typical of the genus Aggregatibacter. Key phenotypic tests for discrimination of the new cluster from validly named Aggregatibacter species are alanine-phenylalanine-proline arylamidase, N-acetylglucosamine, and-galactosidase. The name Aggregatibacter kilianii is proposed, with PN528 (CCUG 70536T or DSM 105094T) as the type strain.