Bacterial isolation and evaluation of antisepsis protocols of the operative field of bitches submitted to ovariohysterectomy

Abstract

This study evaluated the efficiency of five antisepsis protocols performed in the operative field of bitches due to the importance of preventing surgical infections in veterinary medicine. Thirty female bitches submitted to elective ovariohysterectomy (OVH) were used and were separated into groups of eight animals. In group I, 70% alcohol and 2% chlorhexidine gluconate (CHG) were administered; in group II, alcoholic CHG 0.5%; in group III, polyvinylpyrrolidone (PVPI) 10% alcohol; in group IV, alcohol 70% and alcoholic PVPI 10%; and in group V, alcohol, PVPI 10%, and CHG degermante 2%. Samples were collected with sterile swabs before antisepsis, and after 3 min, each product was used and seeded in a specific medium for colony forming unit (CFU) counts. The identification of the isolates was performed according to the morphological, dyeing, and biochemical characteristics, namely, Bacillus spp., Staphylococcus spp., Klebsiella pneumoniae, Klebsiella oxytoca, Shigella sonnei, Shigella spp., Enterobacter aerogenes, Enterobacter spp., Salmonella spp., and Escherichia coli. The results obtained allow us to conclude that the protocols with 0.5% CHG and 2% CHG degermante were the most efficient in antisepsis that reduced 100% of the CFU of the skin and presented better residual power until the end of the surgical procedure.