Molecular and ampelographic characterization of grapevine (Vitis vinifera L.) genetic materials collected in natural flora of ganos mountains

Abstract

This study was carried out to determine the molecular and morphological characterization of the populations of Vitis vinifera L. grapevine forms which have been adapted to the natural flora of the Trakya Region in Ganos (Işiklar) Mountains. Materials were collected from 7 different areas on the northern and southern slopes of the mountains between 2014 and 2016 (May, June and July) used for DNA isolation. In 2014 year, 103 samples were taken from surveys. Molecular characterization was carried out in 2016 year on 34 samples were used which have grape clusters. It has been determined that genotypes are different from each other in terms of ampelographic and molecular properties and basis 5 different clusters. In the molecular characterization of 34 genotypes, it performed on the two basic components, there were 3 different clusters formed in terms of clustering analysis. As a result, grapevine biodiversity with molecular characterization in the region is defined. This will be a reference for the further grapevine breeding studies, and also the identification of genotypes. It will be opportunity to practical use in the future for growing regional cultivars. On the other hand these findings will be used as a reference to comparison with SSR marker studies about grape varieties.