Effect of Genistein on Heat Shock Protein 47 and Collagen Type IV in Diabetic Rat

Abstract

Diabetes nephropathy (DN) is one of the most common complication in Diabetes Mellitus (DM). DN is an inflammatory process which involved immune cells and effect of genistein prevent this mechanism. However, the effects on HSP 47 and collagen type IV are not yet verified. The purpose of this study was to investigate whether the genistein can suppress HSP 47 and collagen type IV.This study is experimental design used 25 rats. Rats were divided into five groups; normal group, hyperglycemia group, hyperglycemia by administering genistein 0.5 mg/kgw, 1mg/kgw, and 2 mg/kg. Streptozotocin induced 65 mg/kg administered intraperitoneal. Treatment duration is 4 weeks. After 4 weeks of blood was collected via the orbital vein and examined the levels of HSP 47 then rats’ kidneys were taken to see the levels of collagen type IV.The average levels of HSP 47 in non diabetic control group was 1.7982 ng/ml, diabetic control 7.9424 ng/ml, STZ; G 0.5 mg/kgw 5.4192 ng/ml, STZ; G 1 mg/kgw 3.1152 ng/ml and STZ; G 2 mg/kgw 1.849 ng/ml, with p value 0.000 (p