Development of a doubled haploid mapping population and linkage map for the bread wheat cross kariega × avocet s

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Abstract

A doubled haploid wheat population of 150 lines was constructed from the F1 of a cross between cultivars Kariega and Avocet S using the wheat-maize technique. Segregation and linkage analysis of 203 DNA markers, two storage protein markers and the stem rust resistance gene Sr26 yielded 31 linkage groups of which 28 were chromosome-anchored. These linkage groups covered the entire genome of 21 chromosomes. SSR markers were advantageous for chromosome anchoring and AFLP markers for consolidation of the various linkage groups. A significant feature of the linkage map is a relatively low level of polymorphism for markers on the D genome, viz, 18.5% of all markers mapped. The Kariega × Avocet S doubled haploid population and linkage maps have emerged as valuable resources for further genetic studies of qualitative and quantitative traits of economic importance in bread wheat. © 2005, Taylor & Francis Group, LLC.

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Prins, R., Ramburan, V. P., Pretorius, Z. A., Boyd, L. A., Boshoff, W. H. P., Smith, P. H., & Louw, J. H. (2005). Development of a doubled haploid mapping population and linkage map for the bread wheat cross kariega × avocet s. South African Journal of Plant and Soil, 22(1), 1–8. https://doi.org/10.1080/02571862.2005.10634672

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