Skin surface proteolytic activity in the living animal was determined by a sensitive, non-invasive methodology developed in our laboratory. A non- leaky well was constructed on the shaved back of an anesthetized guinea pig. The well contained the reaction mixture including the substrate 125I-S- carboxymethylated insulin B-chain (ICMI). The proteolytic activity was shown to be time-dependent. The activity was strongly inhibited by pepstatin A, indicating the involvement of aspartic proteinase(s) such as cathepsin D and/or E. Pretreatment of the skin with propylene glycol blocked the proteolytic activity. The present study demonstrates the presence of proteolytic activity located on skin surface using a unique, non-invasive method for in situ proteinase determination in the living animal.
CITATION STYLE
Wormser, U., Brodsky, B., Moor, E. V., Eldad, A., Gal, R., Nyska, A., & Kohen, R. (1998). Skin surface proteolytic activity: Partial characterization and identification. In Advances in Experimental Medicine and Biology (Vol. 436, pp. 207–212). Springer New York LLC. https://doi.org/10.1007/978-1-4615-5373-1_29
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