Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum

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Abstract

Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes that resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Streptococcus responsible for its hallmark β-hemolytic phenotype. Genetic complementation, in vitro reconstitution, mass spectral analysis, and plasmid intergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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Gonzalez, D. J., Lee, S. W., Hensler, M. E., Markley, A. L., Dahesh, S., Mitchell, D. A., … Dorresteina, P. C. (2010). Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum. Journal of Biological Chemistry, 285(36), 28220–28228. https://doi.org/10.1074/jbc.M110.118554

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