In vitro selection of unnatural cyclic peptide libraries via mrna display

Abstract

The ribosomal synthesis of drug-like peptides containing unnatural amino acids is possible due to the broad substrate specificity of the ribosome. In this protocol, a reconstituted Escherichia coli ribosomal translation system (PURE) is adapted to incorporate unnatural amino acids into mRNA-displayed peptide libraries, which are used in in vitro selection. © 2012 Springer Science+Business Media, LLC.