Production and Optimization of Polyhydroxybutyrate from Rhizobium sp. present in root nodules

Abstract

Bioplastics, a form of plastics derived from renewable biomass such as vegetable oil, corn starch, pea starch or microbiota. The biopolymer polyhydroxybutyrate, polyester produced by certain bacteria during the addition of excess carbon sources. In the present study a gram negative PHB accumulating microbe was isolated and identified. Based on the morphological and physiological properties it was identified as Rhizobium species. The different media parameter such as pH, carbon sources, nitrogen sources and temperature was optimized for increased PHB accumulation. Based on different pH, pH 5 was more suitable for increased PHB accumulation by Rhizobium. According to different carbon and nitrogen sources, glucose and potassium nitrate showed high PHB accumulation. Based on the effect of different temperature 300C was more suitable for increased amount of PHB accumulation. PHB production by this strain was in the stationary phase of their growth. PHB was extracted by chloroform and the extracted polymer was determined by UV-VIS spectrophotometer at 235 nm.