Plants are valuable systems for analyzing the acentriolar mitotic spindle. We have developed methods for imaging the mitotic spindle in living tobacco (Nicotiana tabacum) suspension culture cells expressing GFP-α-tubulin. The methods allow the spindle to be observed in living cells at high spatial and temporal resolution and rely on water immersion objectives, spinning disk optics, and high-sensitivity cameras. Here, we describe these methods and provide step-by-step protocols for certain key steps. We also describe a method for application and removal of inhibitors.
CITATION STYLE
Murata, T., & Baskin, T. I. (2014). Imaging the mitotic spindle by spinning disk microscopy in tobacco suspension cultured cells. Methods in Molecular Biology, 1136, 47–55. https://doi.org/10.1007/978-1-4939-0329-0_4
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