Southwestern blotting is a technique used to study DNA-protein interactions. This method detects specific DNA-binding proteins by incubating radiolabeled DNA with a gel blot, washing, and visualizing through autoradiography. A blot resulting from 1-dimensional SDS-PAGE reveals the molecular weight of the binding proteins. To increase separation and determine isoelectric point a 2-dimensional gel can be blotted. Additional dimensions of electrophoresis, such as a gel shift (EMSA), can precede isoelectric focusing and SDS-PAGE to further improve separation. Combined with other techniques, such as mass spectrometry, the DNA-binding protein can be identified.
CITATION STYLE
Jia, Y., Nagore, L., & Jarrett, H. (2015). Southwestern blotting assay. In Methods in Molecular Biology (Vol. 1334, pp. 85–99). Humana Press Inc. https://doi.org/10.1007/978-1-4939-2877-4_5
Mendeley helps you to discover research relevant for your work.